An Integrated Multi-OMICS Approach Highlights Elevated Non-Esterified Fatty Acids Impact BeWo Trophoblast Metabolism and Lipid Processing

Maternal obesity and gestational diabetes mellitus (GDM) are linked with impaired placental function early onset of non-communicable cardiometabolic diseases in offspring. Previous studies have highlighted that the dietary non-esterified fatty acids (NEFAs) palmitate (PA) oleate (OA), key metabolites associated maternal GDM, potential modulators lipid processing. Using BeWo cell line model, current study integrated transcriptomic (mRNA microarray), metabolomic, lipidomic readouts to characterize underlying impacts exogenous PA OA on villous trophoblast metabolism. Targeted gas chromatography thin-layer saturated monounsaturated NEFAs differentially impact profiles. Furthermore, cellular profiles differed when exposed single multiple NEFA species. Additional multi-omic analyses suggested exposure is enrichment β-oxidation pathways, while anti-inflammatory antioxidant pathways. Overall, this further demonstrated important regulators Encouraging appropriate advice implementing interventions maintain by limiting excessive remain crucial managing at-risk obese GDM pregnancies.